자료유형  

 학위논문

Control Number  

0016934843

International Standard Book Number  

9798380335997

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Ng, Henry.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, San Francisco., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(153 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Johnson, Alexander;Al-Sady, Bassem.

Dissertation Note  

Thesis (Ph.D.)--University of California, San Francisco, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약The genetic material is packaged differentially at different phases of the cell cycle. In interphase, cells package their genome into heterochromatin and euchromatin domains, where genes are repressed or expressed based on cell identity. Heterochromatin, the gene-repressive structure, is regulated by different factors that control methylation and acetylation on histones. During cell division, these histone modifications are copied onto the newly synthesized DNA. The heterochromatin structure is seeded from nucleation DNA sequence that recruits other factors to spread the domain. In our studies, we found that the factors required for spreading heterochromatin in different genomic regions are highly variable. Notably, we proposed a mechanism by which Fkh2, a transcription factor, recruits Clr6 histone deacetylase complex I" to a nucleated heterochromatin domain that initiates the spread of heterochromatin domains.During mitosis, the chromatin is packaged into condensed chromosomes for faithful segregation of the duplicated genome. Progression through mitosis is regulated by the levels of a family of protein kinase complexes named Cyclin-Dependent Kinases (CDKs). The phosphorylation of numerous CDK substrates drives various mitosis events. We identified a novel phosphate-binding pocket on cyclins that aids in the timing of sequential multisite phosphorylation in CDK substrates and mitotic events. Loss of this pocket causes a mitotic delay in vivo as well as loss of multisite phosphorylation in various CDK substrates in vitro. 

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Genetics.

Index Term-Uncontrolled  

Heterochromatin

Index Term-Uncontrolled  

Mitosis

Index Term-Uncontrolled  

Phosphorylation

Index Term-Uncontrolled  

Cell cycle

Index Term-Uncontrolled  

Genetic material

Added Entry-Corporate Name  

University of California, San Francisco Biochemistry and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:639243