Material Type  

 학위논문

 

0016930934

Date and Time of Latest Transaction  

20240214095838

ISBN  

9798380210645

DDC  

636

Author  

Dickson, Mackenzie Joy.

Title/Author  

Long-Term Impacts of Uterine Infection on Fertility in Dairy Cows - [electronic resource]

Publish Info  

[S.l.] : University of Florida., 2021

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2021

Material Info  

1 online resource(278 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Bromfield, John J.

학위논문주기  

Thesis (Ph.D.)--University of Florida, 2021.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Abstracts/Etc  

요약In cows, uterine disease is associated with infertility after disease resolution. Cows with uterine disease have reduced estradiol secretion, decreased conception rates, and increased pregnancy loss. The mechanisms responsible for this persistent infertility are unknown. The bacterial component, lipopolysaccharide (LPS), accumulates in the follicular fluid of cows with uterine disease, potentially altering the follicular environment in which oocytes develop. In vitro, LPS decreases oocyte competence and reduces CYP19A1 expression and estradiol production in granulosa cells. Embryo transfer does not restore fertility in cows after uterine disease, suggesting uterine disease impacts the endometrium.I hypothesized that induced uterine disease reduces the developmental competence of oocytes and alters the endometrial signature of pregnancy. Furthermore, I hypothesized that CCAAT/Enhancer-binding protein (CEBPβ), which regulates CYP19A1 expression, mediates LPS-induced downregulation of CYP19A1 expression and estradiol production in granulosa cells.Cows received an intrauterine infusion of pathogenic bacteria or vehicle to induce uterine disease. Subsequently, oocytes were collected and subjected to in vitro fertilization and embryo culture. Bacterial infusion resulted in reduced embryo development compared to vehicle infused controls.RNA sequencing was performed on endometrial tissue on day 16 after insemination in cows that received an intrauterine bacterial infusion. The endometrial signature of pregnancy in cows following bacterial infusion was compared to the endometrial signature of pregnancy previously reported in healthy cows. Compared to healthy cows, the endometrial signature of pregnancy in cows following bacterial infusion showed a dysregulation of pathways related to inflammation and immune function, including IL-7, TLR, and iNOS signaling.Lastly, I demonstrated that LPS reduces estradiol secretion in bovine granulosa cells from small/medium and large follicles. Interestingly the data suggests that LPS mediated changes to CYP19A1 expression and CEBPβ nuclear localization in granulosa cells is dependent on follicle size.In summary, intrauterine bacterial infusion reduces oocyte competence and results in a unique endometrial transcriptome of cows that fail to become pregnant, while granulosa cells exposed to LPS alter CEBPβ signaling and reduce estradiol secretion. Collectively, these studies illuminate potential mechanisms in the ovary and endometrium for the persistent infertility observed in dairy cows after the resolution of uterine disease.

Subject Added Entry-Topical Term  

Animal sciences.

Subject Added Entry-Topical Term  

Cellular biology.

Index Term-Uncontrolled  

Bovine granulosa cells

Index Term-Uncontrolled  

Infertility

Index Term-Uncontrolled  

Inflammation

Index Term-Uncontrolled  

Ovary

Index Term-Uncontrolled  

Uterus

Added Entry-Corporate Name  

University of Florida Animal Molecular and Cellular Biology

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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소장사항  

202402 2024

Control Number  

joongbu:639169