Material Type  

 학위논문

 

0014996579

Date and Time of Latest Transaction  

20190102171202

ISBN  

9780438024175

DDC  

574

Author  

Carrocci, Tucker Joe.

Title/Author  

Biochemical and Genetic Characterization of Factors that Influence Branchsite Selection during Pre-mRNA Splicing in Yeast

Publish Info  

[Sl] : The University of Wisconsin - Madison, 2017

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2017

Material Info  

268 p

General Note  

Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.

General Note  

Adviser: Aaron A. Hoskins.

학위논문주기  

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017.

Abstracts/Etc  

요약RNA splicing plays many critical roles in eukaryotic gene expression, yet even 40 years after its discovery, the mechanism by which the spliceosome recognizes and removes introns is still poorly understood. In my thesis, I describe methods to pr

Abstracts/Etc  

요약In my thesis, I discuss methods to label RNA fluorescently. I used MS2 fusion proteins tags to the label RNAs in living yeast using small molecule fluorophores. These tags allow for visualization of localized RNAs and demonstrate that organic fl

Abstracts/Etc  

요약In the second portion of my thesis, I discuss work on the role of splicing factors that contribute to branchsite choice. I explored the role of cancer causing mutations in a protein called SF3b1 using the yeast spliceosome. Mutations of SF3b1 re

Abstracts/Etc  

요약Finally, I used smFRET to observe dynamics in the splicing factor Prp5, which ensures proper branchsite usage by the spliceosome. I showed that Prp5 primarily adopts an open state in the absence of ATP and RNA but transitions to a closed state u

Subject Added Entry-Topical Term  

Biochemistry

Added Entry-Corporate Name  

The University of Wisconsin - Madison Biochemistry-ALS

Host Item Entry  

Dissertation Abstracts International. 79-10B(E).

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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소장사항  

201812 2019

Control Number  

joongbu:557785